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Protocols

Protocols module of the Life Science Network enables sharing of detailed experimental protocols, laboratory recipes and data analysis procedures.

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External protocol30 Apr 2014

Protocol for lentiviral knock down in mouse ES cells

by W.W.M. Pim Pijnappel, Marijke P.A. Baltissen, and H.T.Marc Timmers
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External protocol30 Apr 2014

Ruthenium hydroxide supported on magnetic nanoparticles: a benign aqueous protocol for hydration of nitriles

by Vivek Polshettiwar and R. S. Varma
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External protocol03 May 2014

Lysine decarboxylase assay with cucurbituril (cucurbit-7-uril)

by Andreas Hennig, Hüseyin Bakirci, and Werner M. Nau
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External protocol03 May 2014

Studying effect of hypergravity on cleavage timings in developing embryos of Limnaea

by Abhishek D. Garg, Dhananjay D. Garg, and Rajshekhar V. Hippargi
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External protocol30 Apr 2014

VINCULIN TAIL (878-1065aa, Wt and Y1064E mutant), expression and purification

by Robert Liddington and Andrey Bobkov
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External protocol30 Apr 2014

Isothermal titration calorimetry to determine the association constants for a ligand bound simultaneously to two specific protein binding sites with different affinities

by Giorgio Pochetti and Roberta Montanari
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External protocol03 May 2014

TH17 cells contribute to uveitis and scleritis and are inhibited by IL-27/STAT1 in the retina (1) Detection of TH17 cells in human blood

by Charles Egwuagu, Cheng-Rong Yu, Ahjoku Amadi-Obi, Xuebin Liu, Rashid Mahdi, and Yun Sang Lee
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External protocol30 Apr 2014

Radioisotopic Assay of L-carnitine (CN)

by Wei Zou
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External protocol03 May 2014

TH17 cells contribute to uveitis and scleritis and are inhibited by IL-27/STAT1 in the retina. (4) Confocal microscopy

by Charles Egwuagu, Cheng-Rong Yu, Ahjoku Amadi-Obi, Xuebin Liu, Rashid Mahdi, and Yun Sang Lee
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External protocol30 Apr 2014

Identification of protease cleavage-sites in E. coli lysate by differential N-terminomic analysis

by John C. Timmer and Guy S. Salvesen
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