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Protocols

Protocols module of the Life Science Network enables sharing of detailed experimental protocols, laboratory recipes and data analysis procedures.

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External protocol30 Apr 2014

Protocols to examine integrin binding with Talin and protocol to test integrin activation by talin

by Feng Ye and Mark Ginsberg
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External protocol30 Apr 2014

Total Phospholipids and Phospholipid Subclasses Determination

by Wei Zou
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External protocol30 Apr 2014

VINCULIN TAIL (878-1065aa, Wt and Y1064E mutant), expression and purification

by Robert Liddington and Andrey Bobkov
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External protocol03 May 2014

TH17 cells contribute to uveitis and scleritis and are inhibited by IL-27/STAT1 in the retina. (6) Retinal cell isolation

by Charles Egwuagu, Cheng-Rong Yu, Ahjoku Amadi-Obi, Xuebin Liu, Rashid Mahdi, and Yun Sang Lee
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External protocol30 Apr 2014

A bioinformatics protocol for rigorous structure-based sequence alignment of distantly related proteins

by Karuppiah Kanagarajadurai, S Kalaimathy, Paramasivam Nagarajan, and Ramanathan Sowdhamini
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External protocol30 Apr 2014

Preparation of mineralised matrices secreted from human primary osteoblasts for analysis of the interaction of cancer cells and the bone microenvironment

by Johannes C. Reichert, Les J. Burke, Judith A. Clements, and Dietmar W. Hutmacher
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External protocol03 May 2014

Mitpred2: an improved method for predicting mitochondrial proteins using SVM and HMM

by Manish Kumar, Ruchi Verma, and Gajendra Raghava
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External protocol30 Apr 2014

IRMPD-based quantification of ER-catalyzed saturation and cyclopropanation reactions

by Liangcai Gu, Bo Wang, David Sherman, and Kristina Håkansson
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External protocol10 Apr 2014

Tumor cell-collagen interactions: Identification and semi-quantitative evaluation of selectively-expressed genes by combination of differential display- and multiplex-PCR

by Rosalia Sirchia, Valentina Ciacciofera, Claudio Luparello
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External protocol30 Apr 2014

Quantification of Saccharomyces cerevisiae pentose-phosphate pathway intermediates by LC-MS/MS

by Mirjam Wamelink, Erwin Jansen, Eduard Struys, Hans Lehrach, Cornelis Jakobs, and Markus Ralser
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