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Protocols

Protocols module of the Life Science Network enables sharing of detailed experimental protocols, laboratory recipes and data analysis procedures.

You can upload a protocol here

Experimental protocol09 Oct 2015

Protocol for Cloning of CRISPR Genome Engineering Constructs

by Feng Zhang
- Views 4044
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Experimental protocol29 Oct 2013

Western blot protocol in 7 different languages (French, German, Greek, Italian, Polish, Russian, English)

by Mark Livingstone
- Views 3945
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Experimental protocol13 Mar 2013

Application of membrane-permeant PIP3/AM to cells

by Vibor Laketa
- Views 3527
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Experimental protocol28 Oct 2015

Metabolic Assays for Detection of Neutral Fat Stores

by Jan M. Baumann, Leila Kokabee, Xianhiu Wang, Yan Sun, Jason Wong and Douglas S. Conklin
- Views 3032
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Experimental protocol09 Oct 2015

Agarose gel electrophoresis

by OpenWetWare
- Views 2776
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Recipe19 Mar 2013

Rapamycin reconstitution

by Vibor Laketa
- Views 2635
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Experimental protocol27 Dec 2015

Glucose Production Assay in Primary Mouse Hepatocytes

by Michihiro Matsumoto* , Mashito Sakai
- Views 2315
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External protocol29 Oct 2016

Autophagy Assays (LC3B immunofluorescence, LC3B western blot, acridine orange assay)

by Xin Zhang and Qingsong Liu
- Views 2148
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Recipe13 Mar 2013

Hepatocyte growth factor (HGF) reconstitution

by Vibor Laketa
- Views 1960
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Experimental protocol15 Mar 2013

Cell seeding for semi-automated imaging (reverse transfection)

by Alen Piljic
- Views 1534
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