About
Positions
Principal Investigator Sep 2021 -
Postdoctoral fellow Jun 2019 - Jun 2020
Postdoctoral Research Scientist May 2017 - Apr 2018
Postdoctoral Researcher Oct 2016 - Apr 2017
PhD Student/ Scientist Aug 2011 - Sep 2016
Education
Jagiellonian University, Cracow, Poland
Field of study: Biology
Degree: Master of Science
Technical University Munich, Germany 2011 - 2016
Field of study: Biomedical sciences
Degree: PhD
Skills
Isolation and culture of murine intestinal crypts in 3D culture system, isolation and culture of human esophageal tumor cells in 3D cultures system, isolation and culture of mouse and human fibroblasts, molecular biology, immunohistochemistry, trogocytosis assay, Western blot, Saccharomyces cerevisiae, urogenital sinus explant culture, stromal-epithelial co-cultures, confocal microscope
Professional interests
Tumor microenvironment, adult stem cell niche, metastatic niche, mechanisms of cancer cell metastasis, disseminated tumor cells, basic and translational cancer research, cellular stress responses, 3D cell biology
Languages
Polish (native), English (fluent), German (B2), Spanish (A1)
Additional information
I am a biologist interested in both basic and translational cancer research. Since 2011 I have been doing doctorate at medical department, Klinikum rechts der Isar TUM. In my PhD project I study mesenchymal-epithelial cross-talk in the intestinal stem cell niche. My research model involves several three dimensional cell culture systems: crypt culture (1, 2), organotypic cell culture (3) and tissue culture ex vivo (4). In my project I address the question: How proliferation and differentiation of intestinal stem cells is regulated by niche? In parallel I am working on translational project aimed at the establishment of human-derived organoids from Barrett´s Esophagus and esophageal adenocarcinoma biopsies.
References
1. Sato T, Vries RG, Snippert HJ, van de Wetering M, Barker N, Stange DE, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature. 2009;459(7244):262-5.
2. Pastula A, Quante M. Isolation and 3-dimensional Culture of Primary Murine Intestinal Epithelial Cells. BioProtocol. 2014.
3. Kalabis J, Wong GS, Vega ME, Natsuizaka M, Robertson ES, Herlyn M, et al. Isolation and characterization of mouse and human esophageal epithelial cells in 3D organotypic culture. Nature protocols. 2012;7(2):235-46.
4. Ootani A, Li X, Sangiorgi E, Ho QT, Ueno H, Toda S, et al. Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche. Nature medicine. 2009;15(6):701-6.