Characterization and Optimization of Streptomyces albidoflavus MD102 as a heterologous expression chassis

Authors

Lee, S. Q. E., Candra, H., Ma, G.-L., Liang, Z.-X.

Abstract

We report the isolation and characterization of Streptomyces albidoflavus MD102, a strain that can be used as a microbial chassis for the heterologous production of secondary metabolites. This strain, closely related to the widely used S. albidoflavus J1074, exhibits a compact genome, exceptional genetic tractability, rapid growth, and susceptibility to antibiotics. Whole-genome sequencing revealed the metabolic capabilities of S. albidoflavus MD102, highlighting its versatility in supporting the production of diverse secondary metabolites. Employing CRISPR/Cas9-assisted genome editing tools, we created mutant strains with reduced genome and cleaner chromatographic background. In addition to the deletion of several biosynthetic gene clusters (BGC), we inserted the global regulator bldA gene and geranyl diphosphate synthase (gpps) genes and an additional {Phi}BT1-attB attachment site into the chromosome to enhance the strain's capability in producing secondary metabolites. S. albidoflavus MD102 will be a new addition to the repertoire of existing Streptomyces chassis, contributing to the advancement of secondary metabolite discovery and synthetic microbiology.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 02 Mar 2026.

Stats

1-terrible, 9-excellent. How would you rate this preprint? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.