Authors
Kip, A. M., Carvalho, D., Nazzari, M., Romitti, M., Waddington, J., Branca, C., Bryan, C., Jutten, B., van de Vin, W., Patel, P., Poulsen, R., Hansen, M., Thomas, S., Pennington, S., Costagliola, S., Caiment, F., Giselbrecht, S., Moroni, L.
Abstract
Endocrine disrupting chemicals (EDCs) are ubiquitous environmental contaminants capable of dysregulating the production of thyroid hormones. Traditional thyroid toxicological assays rely on 2D cell cultures and animal models, both of which fail to accurately recapitulate human thyroid physiology and provide limited mechanistic insight into EDC toxicity. To overcome these limitations, we report a novel thyroid on chip platform integrating mouse embryonic stem cell derived thyroid organoids with advanced organ on chip (OoC) technology and downstream multiomics analysis. The platform leverages a reversibly sealed microphysiological flow battery (MFB) to allow scale up of dynamic organoid culture and controlled chemical exposure while reducing operational complexity. Upon EDC exposure, transcriptomic and proteomic analysis revealed new molecular signatures of thyroid disruption across four different EDC classes, even at very low EDC concentrations (1nM), validating the capacity of this system to mechanistically dissect EDC induced responses. This represents an integrated platform consists of an advanced physiologically relevant assay framework for next-generation endocrine toxicity testing, bridging the gap between in vitro screening and in vivo thyroid physiology.
Preprint server:
bioRxiv
The authors list and abstract were imported from bioRxiv on 12 May 2026.
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