Authors
Moran, S., Fan, Z., Zanovello, M., Feng, F., Wang, X. Q. D., Lu, C., Liu, J., Zhang, X.
Abstract
Polycomb targeted loci form long-range chromatin interactions independent of CTCF-cohesion and are demarcated by low DNA methylation. Polycomb targets form extremely long-range loops (long Polycomb loops) that can separate anchors up to 60 Mb. These loops predominantly occur in cells of self-renewal status such as human hematopoietic stem cells (HSC) and mouse embryonic stem cells (ESC), but rarely in cell lines. To identify long Polycomb loops in cancer, we initiated a pan-cancer survey of long Polycomb loops in a collection of 235 tumor samples (33 acute myeloid leukemias, 17 Breast cancer samples, 63 pediatric brain tumors, 70 prostate cancers, and 42 colon cancers). We found most cancers, including all prostate cancers and colon cancers, lack long Polycomb loops except pediatric brain tumors and certain AMLs. In pediatric brain tumors, we found the ependymoma PFA subtype (which shows globally depleted H3K27me3) notably displayed strong long Polycomb loop interactions. AML displayed more diverse levels of long Polycomb loop interactions. Most AMLs lost both long Polycomb loops with Polycomb binding loss at the loop anchors. Whereas 10% of AMLs retain long Polycomb loops as strong as in HSCs. These AMLs recurrently carry mutations in CEBPA and STAG2, which are not associated with the Polycomb complex or DNA methylation machinery. We found that long Polycomb loop strong AML is sensitive to EZH2 inhibition, which induces cell differentiation. Conversely, PRC1 component dependency can be used to predict long Polycomb loop formation in B-cell lymphoblastic leukemia cell lines. Our analysis suggests that the oncogenesis process antagonizes the long Polycomb loop maintenance in most cancers, yet certain cancers may still preserve strong long Polycomb loops from cell-of-origin. The maintenance of long Polycomb loops sensitizes cells to Polycomb inhibition, indicating that such loops could be an epigenomic biomarker for pharmacological or genetic Polycomb inhibition.
Preprint server:
bioRxiv
The authors list and abstract were imported from bioRxiv on 01 Nov 2025.
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