Tracking multi-site somatic voltage dynamics via high-speed fiber photometry

Authors

Chakraborty, S., van Veghel, M., Tzanou, A., Li, Z., Torbin, D., Lowet, E.

Abstract

Investigating neural circuit dynamics across distributed brain regions in awake, behaving animals is crucial for understanding complex behavior. Genetically encoded voltage indicators (GEVIs) offer a powerful approach to tracking transmembrane voltage with high temporal and cellular specificity. However, scaling high-sensitivity GEVI recordings across multiple brain regions and multiple animals simultaneously remains a major technical challenge. Furthermore, it is unclear whether soma-targeted GEVIs - typically used for single-cell resolution imaging - can be effectively adapted for fiber photometry. Here, we show that a sCMOS-based widefield imaging system achieves sensitive dual-color multi-site fiber photometry using soma-targeted GEVI indicators with high temporal resolution. We validated this approach in the mouse hippocampal CA1, capturing theta (3-10Hz) and gamma (30-80Hz) rhythms and theta-gamma cross-frequency coupling. Additionally, we recorded high-frequency neural entrainment (>100 Hz) and somatic depolarization induced by electrical stimulation in CA1. Lastly, we tracked synchronized neural activity between the bilateral CA1s as well as multi-site dual-color imaging across CA1 and cortex simultaneously in three freely running mice. This work provides a scalable, accessible platform for high-speed optical electrophysiology in distributed neural circuits.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 06 Jun 2026.

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