AKIP1 is an inner scaffold component required for centriole integrity

Authors

Sanada, A., Ito, K. K., Chinen, T., Yamamoto, S., Fukuyama, M., Hata, S., Kitagawa, D.

Abstract

Centrioles are microtubule-based organelles that play crucial roles in various biological processes, including cell division, ciliogenesis and flagellar assembly. These functions require the preservation of centriole structural integrity, yet the molecular mechanisms that maintain this integrity remain incompletely understood. In this study, through large-scale gene co-dependency analysis and ultrastructure expansion microscopy (U-ExM), we identified A-kinase interacting protein 1 (AKIP1) as a novel component of the centriole inner scaffold, a luminal structural framework that supports centriole integrity. Analysis of large-scale functional genomic data from DepMap placed AKIP1 in a functional cluster with the inner scaffold components POC1A, POC1B, and POC5. Consistently, AKIP1 colocalizes with these clustered inner scaffold components in the central core region of centrioles, forming a multicomponent interaction network. We further show that AKIP1 is recruited to centrioles during G2 phase, subsequent to the recruitment of other inner scaffold components, and that its centriolar localization depends on these components. AKIP1 depletion leads to defects in centriolar microtubules and abnormal centriole morphology. Together, our findings establish AKIP1 as a downstream component of the inner scaffold that contributes to the maintenance of centriole integrity.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 09 Jun 2026.

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