Authors
Gabriel, K. A., Davis, O. C., Palomino, S. M., Ishishita, S., Poldsam, H., Brandon, J. M., Inturi, N. N., Mydugolam, H., Khan, I. O., Selvakumaran, N., Shiers, S., Yousuf, M. S., Vines, E., Horton, P., Khan, T., Cervantes, A., Reese, J. C., Patwardhan, A., Dussor, G., Meyers, E., Tavares-Ferreira, D., Pool, A.-H., Price, T. J.
Abstract
The spinal cord is the gateway for sensory information from the body as it ascends to the brain, as well as a major motor output center of the nervous system. It is also a key location for sensory-motor integration, and a processing site for nociceptive information that eventually drives pain perception in the brain. Tremendous progress has been made in understanding spinal cord circuits using genetic and single cell sequencing approaches in mice. Recently, several groups have conducted single-nucleus and spatial sequencing studies in postmortem human spinal cord tissue. However, the spatial properties of spinal cord cellular diversity and potential sex differences that might be important for human physiology remain unexplored. We conducted deep single-nucleus sequencing on dissected lumbar dorsal and ventral spinal cord samples from 11 organ donors, including 6 females and 5 males, and anatomically annotated spinal cord cell types with 10X Xenium single-molecule spatial transcriptomics. We identified 34 spatially and genetically defined neuron classes, many of which have clearly recognizable conserved orthologs in the rodent spinal cord. We also identified sex specific cell types and states within multiple glial types, but not neurons, demonstrating sexual dimorphism at the transcriptomic cell-type level in the adult human spinal cord. The spatial and single-nucleus atlas resulting from our work build upon previous knowledge to better understand human spinal cord physiology and to identify drug targets for neurological diseases affecting the spinal cord, in particular pain.
Preprint server:
bioRxiv
The authors list and abstract were imported from bioRxiv on 02 Nov 2025.
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