Authors
Durak, M. R., Renaud, E., Dutheil, J. Y.
Abstract
Evolutionary rates vary widely among sites in protein sequences, reflecting differences in functional constraints across residues. Individual sites can also experience lineage-specific shifts in substitution rates--a process known as heterotachy--when selective pressures change during evolution. Although such temporal variation has long been recognized, the mechanisms underlying lineage-specific rate shifts and the factors shaping their distribution across protein families remain poorly understood. Here we map site-specific rate shifts across thousands of orthologous protein families spanning the tree of life. Among more than 1.8 million aligned amino-acid sites, over one quarter show evidence of lineage-specific rate changes. Rate shifts are more frequent in families with deep evolutionary origins, including those tracing back to LUCA, whereas younger clade-specific families generally show lower proportions of rate-shifting sites. We next examined whether local structural features predict where rate shifts occur. Residue burial shows only a weak association with rate-shift probability, and its direction differs across domains, with buried residues enriched for rate shifts in Archaea but surface-exposed residues showing slightly higher probabilities in Eukaryota. Moreover, rate-shifting sites rarely form spatial clusters within protein structures, indicating that structural constraints do not globally determine their locations. Despite their widespread occurrence, rate-shifting sites have limited impact on phylogenetic reconstruction beyond random site variation. Together, these results show that lineage-specific rate shifts are a pervasive feature of protein evolution shaped primarily by evolutionary ancestry and phylogenetic depth.
Preprint server:
bioRxiv
The authors list and abstract were imported from bioRxiv on 20 Jun 2026.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 2
- Comments 0