A simple procedure to demonstrate antimicrobial activity in cell-free supernatants

Authors

Zunjarrao, D., Reshamwala, S. M. S.

Abstract

Probiotics produce antimicrobial peptides and small molecules that are secreted into the medium. Antimicrobial activity of cell-free supernatants can be tested using various qualitative and quantitative methods. Many of these techniques employ methods which introduce uncontrolled variables, impacting reproducibility and making comparison of reported results difficult. Here, we present a simple procedure for quantitative estimation of antimicrobial activity of cell-free supernatants which overcomes drawbacks of commonly used methods.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 25 Jun 2026.

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