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Cell-type-resolved spatial proteogenomics from matched genome and proteome of the same cells

Created on 11 Jul 2026

Authors

Zwiebel, M., Wahle, M., Stadler, R., Levesque, M. P., Dummer, R., Nordmann, T. M., Mann, M.

Abstract

The genome and proteome of the same cells are rarely measured together, which is especially consequential in cancer, where somatic mutations vary across clones and drive disease. We show that a single standard proteomics extraction tip can retain peptides on-tip after digestion while genomic DNA passes into the normally discarded flowthrough. Combined with Deep Visual Proteomics, flowthrough co-isolation enables cell-type-resolved spatial proteogenomics from archival FFPE tissue, demonstrated in melanoma.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 11 Jul 2026.

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