Mesohabenular glutamatergic regulation of the consequences of uncontrollable stress

Authors

McGovern, D. J., Deming, M., Mills, H., Polatsek, H., Baratta, M. V., Root, D. H.

Abstract

Perceived or volitional control over a stressor is a critical determinant of resilient versus susceptible behavioral phenotypes. Activation of the lateral habenula (LHb) is required for the behavioral changes or learned helplessness effects that follow inescapable, but not escapable, stress. However, the relevant LHb inputs that regulate its mediation of inescapable stress outcomes are not fully elucidated. We find that ventral tegmental area (VTA) glutamatergic axons in LHb are activated by aversive stimuli, and this activation was not altered by prior inescapable stress experience. Similarly, optogenetic activation of VTA glutamatergic axons in LHb resulted in c-Fos expression in LHb neurons that did not depend on a prior inescapable stress experience. Photoinhibition of VTA glutamatergic axons in LHb during each inescapable stress trial prevented social and nonsocial consequences of inescapable stress. We interpret these results such that VTA glutamatergic inputs to LHb report aversive stimuli to LHb, possibly related to current salient aversive experience without regard to prior stress history. Nevertheless, VTA glutamatergic input to the LHb plays an important role in regulating LHb-mediated social and nonsocial consequences of uncontrollable stress.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 24 Jul 2026.

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