Neuropixels 1536 Channel Quad Base probe reveals brain-wide communication underlying flexible sensorimotor sequences

Authors

Chang, Y.-T., Dasgupta, R., Dong, M., Colonell, J., Karsh, B., Cheng, A., Hermans, R., Mahmud-Il, H., Lopez, C. M., Putzeys, J., Welkenhuysen, M., Dutta, B., Graves, A. R., O'Connor, D. H., Harris, T.

Abstract

The brain orchestrates billions of neurons spanning hundreds of regions to drive behavior. High-density electrophysiology has provided insights into this process, but monitoring brain-wide patterns of spiking activity demands new tools. Here we present Neuropixels 2.0 Quad Base, a 1536-channel probe with four times the simultaneous recording capacity of state-of-art Neuropixels 2.0. Using two Quad Base probes, we performed 3072-channel recordings in mice trained to produce complex sequences of goal-directed licks. We decoded behavioral kinematics from neural populations across more than 20 brain regions and identified the spatiotemporal emergence of motor decision signals. The probes high channel count further revealed expanded inter-neuronal functional subnetworks and latent dynamics across multiple areas and features significantly undersampled when channels were subsampled to mimic standard Neuropixels 2.0. Neuropixels 2.0 Quad Base offers a powerful tool to explore neural encoding of cognition and behavior at grand scale.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 29 Jul 2026.

Stats

1-terrible, 9-excellent. How would you rate this preprint? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.