Authors
Sadia, H., Douglas, K. M., Bray, A., Rummel, A., Alam, P.
Abstract
The accurate segmentation of lizard claws is important as they are materially heterogeneous, comprising both bone and keratinous tissue. This study presents a deep learning framework for the automated segmentation of lizard claw tissues, specifically bone and keratin, from CT imaging data. A dataset comprising 14 lizard claws was used in this work, with annotations generated through a superpixel based labeling approach to provide ground truth reference segmentations. To evaluate the effect of spatial context on segmentation performance, both 2D and 2.5D CNN architectures using DeepLabV3 with ResNet-50, ResNet-101, and Inception-ResNet-v2 backbones were investigated, with predictions subsequently reconstructed into three-dimensional volumes for analysis. Performance was assessed using a leave one out cross validation (LOOCV) strategy and evaluated with 3D Dice Similarity Coefficient (DSC), Intersection over Union (IoU), Sensitivity (Recall), 95th Percentile Hausdorff Distance (HD95), and Relative Volume Error (RVE). Experimental results demonstrate that 2.5D CNN architectures consistently outperform their 2D counterparts across all evaluation metrics, highlighting the importance of incorporating inter-slice contextual information for volumetric tissue segmentation. From amongst the models, the 2.5D Inception-ResNet-v2 achieved the best overall performance, reaching a validation accuracy of 97.5% and producing segmentation results that closely align with ground-truth tissue structures. Our findings demonstrate the effectiveness of 2.5D deep learning approaches for the high accuracy segmentation of heterogeneous lizard claw tissues from CT data, whilst providing a robust framework for automated morphological analysis in comparative anatomical studies.
Preprint server:
bioRxiv
The authors list and abstract were imported from bioRxiv on 29 Jul 2026.
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