Analog intrinsic recoding measures RNA dynamics without chemical conversion

Authors

Hansen, L. N., Couvillion, M. T., McShane, E., Azbukina, N., Treutlein, B., Churchman, L. S.

Abstract

Steady-state RNA abundance measurements mask the synthesis and decay rates that shape gene expression. Analog intrinsic recoding sequencing (AIR-seq) repurposes the base-pairing properties of N4-hydroxycytidine (NHC) to mark newly synthesized RNA with C-to-T and T-to-C mismatches in standard RNA-seq libraries, eliminating chemical conversion and enrichment common to RNA metabolic labeling methods. NHC-AIR-seq resolves bulk and single-cell RNA dynamics, improves RNA velocity inference, reveals hidden regulation, and brings RNA kinetics into routine transcriptomic workflows.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 29 Jul 2026.

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