Plug-and-Play 3D localization microscopy

Authors

Cohen, O. R., Xiao, D., Kedem, R. O., ALALOUF, O., Prakash, J., NAKATANI, Y., Gustavsson, A.-K., Shechtman, Y.

Abstract

Point-spread-function engineering by depth-encoding phase masks enables volumetric super-resolution imaging by 3D single-molecule localization microscopy (SMLM) but usually requires cumbersome relay optics. We demonstrate simple 3D SMLM implementation by phase mask insertion directly into the infinity space of a commercial microscope, along with appropriate computational compensation for field-dependence. The entire inserted component, including the phase mask, is 3D printing-based.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 31 Jul 2026.

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