Coculture of Collinsella aerofaciens and Bacteroides thetaiotaomicron under bile acid stress reveals vitamin B6 exchange

Authors

Wang, Y., Haange, S.-B., Gamarra, A. M., Lenicek, M., Maier, D., Plail, R., Seidl, H., Kaleta, C., Rolle-Kampczyk, U., Jehmlich, N., von Bergen, M.

Abstract

Although bile acid-mediated microbiome-host interactions are known to shape gut microbial composition and function, the mechanisms by which bile acid stress influences microbial metabolic interactions remain poorly understood. Here, we investigated the interaction between two key gut microbes, Bacteroides thetaiotaomicron and Collinsella aerofaciens, under deoxycholic acid (DCA) stress using anaerobic coculture and proteomic analysis. We found that C. aerofaciens mitigated the inhibitory effects of DCA on B. thetaiotaomicron, primarily through DCA uptake from the medium, as confirmed by DCA quantification. Proteomic analysis further showed that DCA broadly disrupted amino acid and vitamin metabolism, particularly in B. thetaiotaomicron, whereas coculture promoted widespread metabolic activation in C. aerofaciens, including enhanced vitamin B6 metabolism and increased production of citrulline and ornithine. These findings suggest that metabolic cooperation enhances resistance to bile acid stress and may contribute to gut microbiome resilience, with potential relevance to liver- and bile acid-related disorders.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 05 Aug 2026.

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