Single shot low-dose radiation durably and focally increases cortical excitability: a potential therapy for neuronal circuit disorders?

Authors

Fan, W., Meier, J., Fu, T., Langenbahn, F., Peter, F., Altahini, S., Cleppien, D., Hehlgans, S., Anthes, J., Schneider, M. B., Wu, H., Adler, J. R., Schmeisser, M. J., Roedel, F., Stroh, A.

Abstract

Herein, we assess the potential of low-dose stereotactic radiosurgery (SRS) to modulate neuronal network states without apparent damage to cellular integrity. Using a small animal radiation research platform (SARRP), a 1 mm3 focal target in the mouse visual cortex was irradiated with doses of 5, 20, and 40 Gy. One-month later a significant dose-dependent increase in excitatory synapse numbers was observed, notably limited to the treated visual cortex and not the adjacent somatosensory cortex. Six months post-irradiation, cortical neuronal microcircuit activity was monitored in awake mice using high sensitivity two-photon calcium imaging. A single 5 Gy dose resulted in a significant microcircuit-wide increase of spontaneous neuronal activity, consistent with a lasting shift in the functional architecture of the irradiated nodal network. At higher SRS doses (40 Gy) this neuromodulatory window appears to close. In aggregate, these data suggest that low-dose radiation could, in some circumstances, be exploited by selected high precision SRS technologies to durably modulate neuronal circuit disorders. Some, or even all the clinical benefits reported in the companion article by Zhao et al. are likely attributable to the biological properties we sought to characterize in our research.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 12 Aug 2026.

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