Size Matters: Small Cell Variants of Coxiella burnetii Initiate Replication Early in Primary Macrophages

Authors

Sim, L. A., GrandPre, P. A., Reed, S. C. O., Case, E. D. R.

Abstract

Coxiella burnetii alternates morphologies to survive in two niches: the external environment and a degradative intracellular compartment. The small cell variant (SCV) is adapted for environmental persistence and transmission of Q fever to ruminants and humans. The large cell variant (LCV) is intracellular, and despite not being a major source of transmission, is infectious in vitro. When modeling infection, researchers typically apply a mixed population of these cell types as inocula. As this practice does not mimic natural infection, it may confound our understanding of early Q fever infection events. We separated SCV and LCV by density gradient centrifugation and compared their replication in primary murine macrophages and a fibroblast cell line. SCV inocula replicated more efficiently than LCVs in both host cell types. LCV replication was delayed for four days in macrophages compared with SCV inocula, which had completed logarithmic growth by that time point. We found no difference in pathogenic vacuole size, but there was a modest difference in their respective bacterial burdens. Interestingly, IL-6 and CXCL2 secretion was significantly elevated in LCV-infected macrophages as compared to SCV at 24 hours, suggesting a difference in the host response to each. This is the first study to demonstrate that C. burnetii developmental status influences the progression of infection.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 20 Aug 2026.

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