Rho/ROCK-dependent actomyosin contractility drives extracellular vesicle release from the cilium

Authors

Spencer, W. J., Kreitman, M. J., Schneider, N. F., Hanke-Gogokhia, C., Finkelstein, S., Ball, D. G., Mitev, P. R., Pazour, G. J., Arshavsky, V. Y.

Abstract

The release of extracellular vesicles (EV) from the primary cilium is a conserved process observed in many cell types. It serves as a rapid and efficient mechanism to release select proteins from the cilium, which can be used for either intercellular communication or membrane material disposal. Previous studies have shown that the release of EVs from the cilium relies on the actin cytoskeleton and proposed several molecular mechanisms that may perform this function. Using the model of IMCD3 cells, we now demonstrate that this process relies on actomyosin contractility supported by non-muscle myosin IIA acting downstream of the RhoA-ROCK signaling pathway. We further showed that the cilia of these cells release EVs independently of de novo actin polymerization, which we confirmed using an in vivo model of mutant photoreceptor cells that release massive amounts of vesicles from their cilia instead of elaborating into light-sensitive outer segment membrane structures.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 26 Aug 2026.

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