Meso2EM: a cross-scale CLEM workflow linking mesoscale functional imaging to targeted electron microscopy

Authors

Oomoto, I., Murate, M., Sohn, J., Tamura, M., Hatada, S., Egawa, N., Odagawa, M., Suga, M., Kawaguchi, Y., Murayama, M., Kubota, Y.

Abstract

Meso2EM is a correlative light and electron microscopy workflow that transfers neurons selected from mesoscale functional images to targeted electron microscopy. We recorded Ca{superscript 2} signals from layer 2/3 neurons across a contiguous 3 x 3 mm cortical field in awake mice and reidentified a selected neuron after fixation and tangential sectioning. Lectin-labeled vascular architecture served as a shared landmark across in vivo two-photon imaging, confocal microscopy, laboratory micro-CT of resin-embedded tissue, and block-surface scanning electron microscopy, guiding focused-ion-beam scanning electron microscopy to the target cell body. The same progressive-targeting principle also supported serial ATUM-SEM reconstruction of an in vivo-tracked dendrite and serial transmission electron microscopy of optically selected dendrites from a patch-clamp-recorded Martinotti cell. Meso2EM therefore provides a practical route for preserving target identity across large changes in scale and specimen state while restricting electron-microscopy acquisition to a selected region.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 02 Sep 2026.

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