Electrical Stunning does not affect Brain Tissue Integrity and Stress Response in Larval Zebrafish

Authors

Burkhardt, D.-S., Schultz, K., Glaser, M., Krais, S., Maile, D., Koehler, H.-R., Triebskorn, R., Arrenberg, A. B.

Abstract

Electrical stunning is a humane euthanasia method for larval zebrafish, and a suitable euthanasia protocol has recently been established. Here, we assess the associated stress response and tissue integrity. When compared to MS-222 overdose and hypothermia, electrical stunning caused no significant Hsp70 increase. Brain tissue was preserved and muscle tissue affected only slightly. Our results support the suitability of electrical stunning for humane euthanasia and artifact-free post-mortem analyses.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 08 Sep 2026.

Stats

1-terrible, 9-excellent. How would you rate this preprint? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.