Single-molecule imaging of DNA repair and cytoplasmic rigidification in intracellular bacteria

Authors

Sargison, F. A., Ducrot, N. C., Kummerer, C., Qu, C., O'Hagan, T. B., Haywood, E., Kudhail, A., Moores, A. N., Baker, L., Uphoff, S.

Abstract

DNA damage is an important component of the antibacterial response of phagocytes, but which DNA repair mechanisms are active in intracellular bacteria remains unclear. We developed a live-cell single-molecule tracking approach to directly measure DNA repair activity in Escherichia coli within macrophages. Phagocytosis activates bacterial base excision and nucleotide excision repair pathways and increases DNA mismatch repair foci indicative of DNA replication errors. Phagocyte-generated stresses also cause a general slowdown in protein diffusion within bacteria, consistent with a transition of the cytoplasm towards a glass-like state, which is associated with reduced metabolic activity. At the single-cell level, DNA repair activity is highly heterogeneous, with metabolically inactive bacteria showing the greatest engagement of repair proteins. Together, these findings reveal how distinct DNA repair pathways are deployed during macrophage infection and link repair activity to the metabolic and biophysical states of individual intracellular bacteria.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 18 Sep 2026.

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