Fully Two-Photon-Driven RESOLFT Microscopy for Super-Resolution Imaging inside Tissue

Authors

Ozaki-Noma, R., Ishii, H., Wazawa, T., Sakamoto, J., Kozawa, Y., Kato, Y., Mukai, E., Nemoto, T., Nagai, T.

Abstract

Two-photon excitation (2PE) microscopy reaches deep into tissue but remains diffraction-limited, and existing 2PE-STED and 2PE-RESOLFT schemes still rely on single-photon depletion or photoswitching. Here we present 2P-RESOLFT microscopy, in which excitation and reversible photoswitching are both two-photon-driven. Using the positive photoswitchable protein Padron2, we achieved 205-nm lateral resolution and resolved previously unreported membrane protrusions of {beta} cells inside pancreatic pseudoislets.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 20 Sep 2026.

Stats

1-terrible, 9-excellent. How would you rate this preprint? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.