CytosMPS: an automated multimodal pooled screening platform for protein, morphology and transcriptomics

Authors

Lopez, T., Rabalais, J., Song, B., Boddicker, A., Schwartz, A., Honigfort, D., Zhao, J., Kellinger, M., Kruglyak, S.

Abstract

Cytos Multimodal Pooled Screening (CytosMPS) measures CRISPR guide identity, morphology, native 3' transcriptome, and protein abundance and localization in the same fixed cells. Following IL-1{beta} stimulation, cells with higher nuclear NF-{kappa}B show lower phospho-p38/HSP27 and higher NFKBIA/DUSP1 in the same cell, a measurement neither imaging nor transcriptomics alone provides. TGFBR2 and CTNNB1 perturbations produce distinct multimodal phenotypes. Guide representation and perturbation transcriptional responses agree with an orthogonal single-cell transcriptomic platform.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 25 Sep 2026.

Stats

1-terrible, 9-excellent. How would you rate this preprint? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.