Mouse Neural Organoids Model the Mature In Vivo Synaptic Proteome

Authors

Giandomenico, S. L., van Oostrum, M., Waselenchuk, Q., Dalla Costa, I., Langer, J. D., Schuman, E. M.

Abstract

Neural organoids represent a promising approach for modelling neural circuits and neurological disease, yet their utility depends on whether they can recapitulate the molecular complexity of mature brain synapses. Here, we optimized a protocol to generate mature forebrain organoids from mouse embryonic stem cells and used time-resolved proteomics to define the emergence of synaptic proteins during maturation. Using fluorescence-activated synaptosome sorting coupled with mass spectrometry, we quantified the synapse-enriched proteome and found a high correlation with matched in vivo synaptic proteomes. These findings demonstrate that complex mature synaptic molecular architecture is present within an in vitro organoid environment.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 27 Sep 2026.

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