The anterior insular cortex mediates higher-order threat learning and memory

Authors

Namkung, H., Mukherjee, B., de Chabot, J., Lockhart, S., Guttman, L., Isehak, I., Clark, A., Saito, A., Miura, I., Ishizuka, K., Kwon, H., Sawa, A.

Abstract

Learning about potential threats is indispensable for survival, and its deficits underlie numerous neuropsychiatric disorders. Human brain imaging suggests the anterior insular cortex (AIC) as a key neural correlate of threat learning, yet its mechanistic role remains unclear. Leveraging multimodal systems neuroscience approaches, we demonstrate that AIC excitatory neurons, specifically layer 5 pyramidal neurons projecting to the basolateral amygdala, are essential for higher-order threat learning/memory. They are specifically required when a predictor is temporally separated from an aversive event or when the predictive information is embedded within a diffuse context. In contrast, the AIC is dispensable for elementary, temporally contiguous threat learning. Surprisingly, while the AIC promotes memory formation, it also constrains the level of fear expression during retrieval in a context-dependent manner. This bidirectional control is mediated by the recruitment of distinct AIC neuronal ensembles. Together, we identify a novel circuit mechanism for bidirectional regulation of higher-order threat learning/memory.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 12 Oct 2026.

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