Single-nucleus chromatin accessibility time course of excisional wound repair in rat skin

Authors

Chai, C., Ding, X., Li, Y., Liang, L., Huang, R., Zhang, W., Li, R., Xie, S., Zeng, X., Zhang, L., Yuan, Y., Wei, X., Wang, J., Wang, S., Ji, S.

Abstract

We present a time-series single-nucleus ATAC-seq dataset from full-thickness excisional wounds in Sprague-Dawley rats. Samples were taken from unwounded skin (normal control) and at 1, 2, 5, 10, and 19 days post-wounding, spanning the inflammatory, proliferative, and remodeling phases of skin repair. We extracted nuclei with a two-step tissue dissociation protocol and prepared libraries using the DNBelab C Series Single-Nucleus ATAC Kit, then sequenced on the MGI DNBSEQ-T1 platform. After stringent quality filtering, 37,555 nuclei passed quality control; the resulting chromatin accessibility profiles cover 23 cell types: fibroblast subpopulations, keratinocytes, vascular endothelial cells, immune cells, and skin appendage cell types. This genome-wide chromatin accessibility dataset is a resource for studying regulatory dynamics during excisional wound repair and for comparing results with other wound healing systems.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 22 Oct 2026.

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