Authors
Qian, L., Dishner, I., Capra, N., Carper, D., Ogbonna, N., Kertesz, V., Cahill, J., Foster, J., Michener, J. K.
Abstract
Polyamides are important natural and synthetic polymers, best exemplified by proteins and nylons respectively. Proteins demonstrate that novel polymers with emergent properties can be generated by combining diverse monomers in precisely defined sequences. However, commercial polyamides represent only a small fraction of the potential diversity in polyamide sequences, due to the synthetic challenges of sequence-controlled polymerization. Amide synthetases have been shown to synthesize a broad array of nylon-relevant diads, but the generation of novel sequenced copolyamides requires enzymes capable of acting with longer and more diverse substrates. In this study, we demonstrated that NRPS-independent siderophore (NIS) synthetases, represented by DesD, can ligate oligomeric substrates. A simultaneous enzyme cascade using DesD enabled the synthesis of an oligotriad directly from unprotected substrates. Moreover, the regioselectivity of DesD allowed the selective synthesis of a sequenced amide tetrad, the precursor to a novel sequenced-defined polyamide with properties superior to nylon 66. This study establishes a direct biocatalytic route for the facile synthesis of sequenced oligoamides from unprotected bifunctional substrates, opening new possibilities to synthesize sequenced copolyamides.
Preprint server:
bioRxiv
The authors list and abstract were imported from bioRxiv on 05 Nov 2025.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 41
- Comments 0