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Multimodal non-ionizing quantitative characterization of enamel hypomineralization

Created on 28 Jan 2026

Authors

Chin, B., Thapa, D., Neshatian, M., Abrams, S., Ghaderi Moghadam, H., Dos Santos, P. H., Casas, M., Mandelis, A., Bozec, L.

Abstract

Enamel hypomineralization is clinically graded but difficult to quantify with visual and radiographic assessment. We evaluated whether optical coherence tomography (OCT) and lock-in thermography imaging (LITI) provide non-ionizing, quantitative lesion localization and phenotyping, using clinical mDDE classification as a practical benchmark and micro-CT (CT) mineral density as an in vitro reference in a subset. Twenty-five extracted first permanent molars were classified as control, hypomineralized (Type 1 white - cream; Type 2 yellow-brown), or other enamel defects. Co - localized OCT A - scan decay slopes and LITI lock-in phase-contrast were measured in predefined ROIs; six teeth underwent CT with hydroxyapatite calibration. Clinical scoring showed substantial agreement (inter-rater {kappa} =0.61; intra-rater; {kappa} =0.70). OCT decay slopes differed across clinical groups (Kruskal - Wallis p=0.00028), separating controls from defect groups, while separation between hypomineralization and other defects was limited. LITI detected thermophotonic hot spots at many clinically identified sites (sensitivity 80%) but showed low specificity (20%) at a single modulation frequency, consistent with high anomaly sensitivity but limited etiologic discrimination. In the CT subset, hypomineralized ROIs showed reduced mineral density relative to adjacent enamel and spatial concordance with OCT and LITI contrast. Together, OCT+LITI support objective lesion mapping and motivate multi-frequency thermophotonics and expanded reference sampling to improve specificity for chairside translation.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 28 Jan 2026.

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