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Uncertainty-Aware Deep Learning for Multi-Metric and Dose-Specific Prediction of Drug Synergy

Created on 08 Nov 2025

Authors

Heydari, M. J., Lye, B., Masouri, P., Marsland, T., Lock, J., McKenna, J., Vafaee, F. G.

Abstract

Accurately predicting drug synergy is critical to accelerate the development of combination therapies for cancer and other complex diseases. Yet, the vast combinatorial drug and dose space poses a substantial challenge, even for modern deep learning approaches. Existing approaches often lack generalisability, collapse rich dose response surfaces into single dose averaged synergy scores, and fail to quantify predictive uncertainty. Here, we introduce AlgoraeOS, a biologically informed, attention-aware deep neural network designed to address these challenges. Trained on the largest harmonised dataset of experimentally tested drug combinations, AlgoraeOS simultaneously predicts multiple synergy metrics, while preserving their empirical correlations and accurately estimating both aleatoric and epistemic uncertainty. The model achieves state-of-the-art performance and strong out-of-distribution generalisability across diverse tissues and drug mechanisms, including rigorous zero-shot and few-shot evaluations. Notably, AlgoraeOS predicts the entire dose-response surface, providing dose-specific inhibition profiles with high precision and scalability to multi-million-point datasets. By integrating uncertainty aware, multi-metric, and dose-resolved prediction into a single unified framework, AlgoraeOS offers a powerful solution for drug combination discovery and establishes a new standard for model development and validation in the field.

Preprint server: bioRxiv
The authors list and abstract were imported from bioRxiv on 08 Nov 2025.

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