Authors
Gor, K., Geissen, E. M., Duss, O.
Abstract
RNA structure starts forming co-transcriptionally as the nascent RNA emerges from the RNA polymerase and is dynamically modulated by cellular factors. How individual RNA conformations, out of an ensemble of RNA molecules, relate to function is not well understood. Here, developing multi-color single-molecule fluorescence microscopy experiments, we track in real-time nascent RNA structure formation, functionally characterizing up to 8 different types of RNA molecules. We find that ribosomal proteins, RNA modification enzymes or antisense oligonucleotides specifically modulate a subset of the RNA folding classes. For example, we provide direct evidence that increased local RNA accessibility at specific sites correlates with the chaperoning activity of ribosomal proteins during ribosome assembly. These experiments provide a general framework to study how dynamic RNA folding, and misfolding, relates to function.
Preprint server:
bioRxiv
The authors list and abstract were imported from bioRxiv on 24 Oct 2025.
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