About
Summary
I am an experienced researcher in the fields of molecular and cellular biology. Graduated in Pharmaceutical Chemistry (University of Bari, Italy), I hold a Ph.D. in Biochemistry and Molecular Biology at the University of the Basque Country (Spain). My practical skills are related to the laboratory environment, I used and can manage techniques of molecular and cell biology, biophysical procedures, and several methods for research in the fields of cancer biology and neurosciences. I am well-versed in study operations, specimen handling, and data interpretation and dissemination. Besides, I matured a great experience in the field of training, teaching, mentoring and I developed the necessary soft skills which made me a serious and responsible professional.
Positions
Senior Postdoc Apr 2016 -
Armenise-Harvard Cancer Biology & Genetics Laboratory, University of Trento
Most of my research and teaching over the years has been focused on the mechanisms of regulation of ion channels, while currently my main research interest is studying the pathological and molecular mechanisms of prostate carcinogenesis
Education
University of the Basque Country (Spain) 2004 - 2010
Field of study: Biochemistry and Molecular Biology
Degree: Ph.D
University of Bari (Italy) 1996 - 2003
Field of study: Chemistry and Pharmaceutical Technology
Degree: M.Sc.
Skills
Cell Biology techniques, cell culture and maintenance of standard cell lines (HEK293T, CHO,…) and other normal and cancer cell lines (prostate, melanoma, brest,…). Establishment, maintenance, and manipulation of 3D cell cultures (organoids, spheroids). Transfection optimization, plasmid transfection, siRNA and shRNA knockdowns. qRT-PCR. Cell-Based Assays in Cancer Research (cytotoxicity, genotoxicity, proliferation, apoptosis, migration, invasion, gene expression, protein expression studies….). Preparation of chemical solutions, reagents and specimens for microscopic examination. Cytology and histology. Immunofluorescence and Immunohistochemistry. Molecular biology techniques, DNA cloning and PCR, DNA and RNA extraction, bacterial cells transformation and culture, media preparation, construction of chimeras and labeling of proteins. Recombinant proteins expression and purification, chromatography (gel filtration, hydrophobic, affinity…), SDS-PAGE, dialysis, protein refolding, native gel electrophoresis (BN-PAGE,…). UV/Vis spectroscopy, fluorescence spectroscopy, dynamic light scattering (DLS), ultracentrifugation, protein pull-down, protein cross-linking, Western blotting, immunoprecipitation, ELISA, gel shift, glycosylation assays. Surface expression assays (chemiluminescent assay of oocytes, biotinylation). Lipids thin layer chromatography (TLC), protein-lipid interaction assays. Sample preparation and notions of HSQC NMR spectroscopy, mass spectrometry and Isothermal titration calorimetry. FRET, Bimolecular Fluorescent Complementation (BiFC). Computer skills: Data and statistical analysis (Excell, Sigma Plot…), PC applications, ImageJ, Paint, MS office, Adobe Photoshop etc.
Keywords: assay development, a talented, skilled and qualified, Bacterial culture, Biochemical assays, Biochemistry , Biology, Biomarkers, Biomedicine, Biophysics, BioTechnology, calcium signaling, Cancer Research, cell and molecular biology, Cell Culture, gel electrophoresis, gene cloning, Grant writing, human tissues, IHC staining, Immunofluorescence (IF) staining, Immunohistochemistry (IHC), Ion channels, , laboratory management, Lentivirus, Molecular Biology, Neuroscience, Oncology, Peer-review, prostate cancer, protein expression, qPCR, research scientist, RT-PCR, sample preparation, Scientific writing, SDS-PAGE, spanish, Tissue culture, training, Translational Research, versatilityProfessional interests
Keywords: Adaptability, assay development, Biochemical assays, Biochemistry , Biomarkers, Biomedicine, Biophysics, BioTechnology, calcium signaling, Cancer Research, cell and molecular biology, cell signaling, Cellular Biology , Clinical Research, Cloning, Gene Expression, Grant writing, Immunohistochemistry (IHC), laboratory management, life science, Molecular Biology, Molecular Medicine, Neuroscience, Pharmacology, prostate cancer, research analyst, research scientist, sample preparationFellowships
Fondazione Umberto Veronesi Postdoctoral fellowship 2016 - 2017
University of the Basque Country Postdoctoral fellowship 2014 - 2016
Fundación Biofísica Bizkaia Postdoctoral fellowship 2013 - 2014
Spanish ion cannel initiative (SICI, Consolider programme) fellowship 2009 - 2012
Basque Country Government fellowship 2004 - 2008
Awards
Extraordinary PhD Award from the University of the Basque Country for the Dissertation: Study of the interaction of calmodulin with KCNQ2 channels 2013
Travel Grant from the Spanish Biophysical Society (SBE) 2012
Best Poster Award. Spanish Biophysical Society (SBE) Meeting. Murcia, Spain 2011
Grants
Starting grant for young researchers, University of Trento 2019 - 2020
Languages
English, Spanish, Italian
CV
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Contact details
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