About
Summary
I did Ph.D. in Biochemistry and subsequently completed postdoctoral research fellowship for two years in the Lab of Molecular Biology and Biotechnology at The University of Tokyo. I gained more than 10 years experiences in conducting molecular biology and biotechnological research, quality assurance, and lab facility management that made me theoretically and technically sound in small molecule screening, DNA barcode analysis, SNP detection, bio-marker development, assay and product development. I am professionally expert in molecular cloning and uncovering transcription regulatory factors in microbial systems using gene expression and protein engineering studies. I am passionate in conducting molecular investigation, modifying and troubleshooting experiments with standardized results that fall within the scope of my routine tasks. The most rewarding achievements of my career at Research Centre for Biochemistry and Food Technology at Yokohama, Japan was screening arrays of myosin heavy chain genes and determination of the distinct types of myosin cDNAs from the fast skeletal muscles of vertebrates, and invented a myosin-specific antibody (patent pending). I am well familiar with practical experience in reviewing FDA and EPA regulations for new chemical/compound selection based on the structural and chemical properties in anti-microbial/concrobium product development. Aside from my molecular biotechnology background, I am highly enthusiastic professional in program coordination, Project Management in R & D, and operation sites. I have proven leadership quality with high interpersonal and negotiation skills, and highly potential in structuring reports, protocols, Standard Operation Procedures (SOPs).
Positions
Researcher Oct 2014 -
Department of Chemistry, University of Toronto
• Performed research project on transcription analysis using plasmid DNA, developed microbial cell-lines for exposure analysis
• Conducted cloning, constructed cDNA, carried out qPCR (Biorad protocol), RNA-Seq
• Designed DNA probes, amplified PCR products, purified and labeled with 32P radio-isotop, quantified radio-activity & DNA-protein binding, validated radiation safety
• Carried out protein expression in E. coli using T-vector-system, purified, characterized targeted protein (gel filtration, FPLC, SDS PAGE, ICP-MS)
• Supervised student’s research projects associated with protein engineering and molecular cloning
• Conducted lab facility management, procurements, Bio-safety, radiation safety management
Research Scientist Oct 2007 - Sep 2011
Research Centre for Biochemistry and Food Technology, FRA
• Conceived novel challenges, received grant for molecular biotechnology & toxicology projects
• Mapped myosin specific antigen, screened myosin-specific antibody
• Concluded ELISA, Western blotting-based myosin detection technique (patent pending)
• Standardized competent cells (JM109, XL1-Blue, Top 10, and DH5α) production protocols
• Performed project on heavy metal-induced gene expression (qPCR), micro-array, SNP detection
• Supervised technical staffs/students, collaborated government and university departments
• Conducted molecular biology and biotechnology lab facility management
Postdoctoral Research Fellow, Japanese Society for the Promotion of Science Oct 2004 - Oct 2006
• Received JSPS collaborative Grant-in-Aid for Scientific Research on protein engineering studies
• Cloned cDNAs, determined primary structure of distinct types myosin heavy chain, isoforms
• Sequenced DNA (ABI 3100), identified functional, hyper-variable regions, solved structure-function of multiple gene clusters, mutational analysis, protein express -T-vector system
• Standardized competent cells (JM109, XL-blue, Top 10, and DHα) production protocols
• Characterized myosin kinetics, thermodynamic properties (DSC)
• Managed lab facilities, procurement, supervised technical staffs/students
Monbukagaku-sho Scholar and Teaching Assistant Apr 2001 - Mar 2004
• Carried out collaborative projects on screening bio-active peptides by enzymatic digestion of underutilized seafood
• Characterized small peptides by column chromatography, quantified, assessed functionality
• Standardized desorption isotherm protocol, elucidated small peptide function on myosin stability, correlation among kinetics, water activity (Aw), and unfrozen water
• Managed lab facility, supervised undergraduate and graduate student, particularly in designing experiment, DSC, HPLC, electrophoretic separation of molecules
Education
School of Science and Technology, Nagasaki University 2001 - 2004
Field of study: Biochemistry: Screening of proteolytic peptides and effect studies on protein structure-function
Degree: Ph.D.
Protein engineering studies and structure-function relationship of myosin from the fast skeletal muscle of aquatic vertebrates and it's human counterparts.
Skills
• Expert in molecular biology laboratory techniques: microbial culture, competent cell preparation, plasmid DNA purification, DNA and RNA extraction, cloning, mutagenesis
• Skilled in cDNA synthesis, PCR and qPCR (Biorad protocol), recombinant DNA methodologies, library construction, DNA sequencing, DNA barcode analysis, SNP detection, genotyping, transcriptome analysis (RNA-Seq)
• Highly experienced in protein engineering, protein purification and characterization: SDS-PAGE, immunoblotting, ELISA, LC-MS, ICP-MS, FPLC, gel filtration, affinity chromatography, immunohistochemistry
• Supervised a variety of technical staffs/students for transcriptional analysis, protein engineering, molecular genetics, and toxicology
• Skilled in technical report writing, patent, manuscript, and grant application, statistical analyses
• Professionally skilled in a variety of DNA sequence editing software, for example, ATGC, Seq. ver.3, BioEdit, etc.
Professional interests
Research and Development Scientist for RNA biology, and transcriptional regulation, protein engineering studies, DNA bar-code analysis, DNA damage and repair mechanism
Fellowships
Japanese Society for the Promotion of Science Fellowship (JSPS) 2004 - 2006
Monbukagaku-sho (MEXT) Research Fellow 2000 - 2004
Grants
The Nickel-Regulated Transcription Factor NikR from H. pylori and E. coli 2014 - 2015
Analysis of methylmercury risk, DNA damage, and detoxification mechanism using Zebrafish as an animal model 2008 - 2011
Development of DNA array technique for species identification 2008 - 2011
Grant in Aid for Scientific Research "Biological nature and statistical modeling of arsenicosis in highly arsenic exposed zone of Bangladesh" 2006 - 2011
Molecular cloning and protein engineering studies of lizardfish myosin 2004 - 2006
Grant in Aid for Scientific Research (Monbukagaku-sho Mext Research Grant): Screening of Proteolytic Peptides from underutilized seafoods and their effect on protein stabilization function. 2001 - 2004
Collaborations
Professor Shugo Watabe 2004 - 2006
Molecular cloning and protein engineering studies of lizardfish myosin.
Professor Kazufumi Osako 2003 - 2005
Technology Development using under-utilized seafood myofibrillar protein
Languages
English, Bengoli, Japanese
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