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Cilli Piera

Ph.D. at Pescara

  • Contribution score: 205
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About

Summary

8 years of expertise in molecular biology, cell biology and biochemistry techniques. Development and validation of experimental protocols. Management and monitoring of different stage of activity research. Monitoring of experimental materials. Ability to contribute to the development of clinical research related document and materials. Good communication skills including the ability to present information to both clinical and non-clinical disciplines. Ability to present scientific data clearly and translate strategies into a work plan. Ability to mentor, train, supervise and inspire confidence in newcomers to the industry.
Ability to work autonomously, also assuming responsibility for projects and structures.Good attitude to teamwork and work experience gained during several years of university training.

Positions

Ph.D. Feb 2015 -

Pescara

Education

University of Rome TRE 2012 - 2015

Degree: PhD in Biology Applied to Human Healt (Molecular and Cell Biology)

Management and monitoring of activity research of different project: 1) Study of
molecular mechanism involved in Trinucleotide repeat expansion (TNR) in a mouse
model for Huntington diseases (R6/2 mice); 2) The multiple role of hMTH1 and an
oxidized dNTPS pool in cancer and neurodegeneration (in vitro and in vivo study); 3)
Involvement of oxidized and unmodified ribonucleotides in TNR progression.

Skills

Capacity and skills acquired in Research Laboratories:
 Strong experience in maintaining and expanding a colony of transgenic mice
and littermate wild-type (5 years of experience)
 Genotyping Transgenic mice by PCR
 Harvesting mouse organ
 Isolation and maintenance of primary microglial cell cultures
 Isolation and maintenance of nervous cell cultures
 Maintenance of Human Carcinoma cell lines, cell separation
 Molecular biology techniques: genomic DNA isolation; Polymerase chain
reaction (PCR); Expression cloning; Macromolecule blotting and probing;
Electrophoretic techniques; Construction and production of vectors for
molecular cloning
 Enzymatic activity assay
 Dosimetry
 Calculation of enzyme kinetics with appropriate software
 Band-shift assay (DNA-protein)
 Immunofluorescence techniques
 Immunoassays
Capacity and skills acquired in Biomedical Laboratories:
 General criteria for accreditation of biomedical laboratories, preventive and
corrective actions
 Informatic systems for the management of analytic data
 Chemical, Hematological, Immunologic, Microscopic and Bacteriological
Diagnostic Analyzes

Ability to work autonomously, also assuming responsibility for projects and structures.
Ability to contribute to the development of clinical research related document and
materials. Development and validation of experimental protocols. Good
communication skills including the ability to present information to both clinical and non-clinical disciplines. Ability to present scientific data clearly and translate strategies
into a work plan. Ability to mentor, train, supervise and inspire confidence in
newcomers to the industry. Management and monitoring of different stage of activity
research. Monitoring of experimental materials. Knowledge of Good Clinical Practice
(GCP), Standard Operating Procedure (SOP) and Case Report Form (CRF).

Collaborations

Istituto superiore di sanità

Study of molecular mechanism involved in Trinucleotide repeat expansion (TNR) in a mouse model for Huntington diseases (R6/2 mice)

Languages

italian, english

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