About
Positions
Postdoctoral research associate Jul 2010 - Jun 2015
- Designed, conducted and coordinated experiments for multiple projects to characterize two recently discovered chains of type VI collagen (Focus of an NIH RO1 grant)
- Managed, trained and coordinated the work of rotating and visiting research staff, including: PhD students, Masters students, volunteer researchers, summer interns, and technicians
- Wrote manuscripts and grants and served as a reviewer of manuscripts
- Contributed ideas and technical expertise for other projects ongoing in the lab and in adjoining labs
- Organized and presented work at lab meetings and also at national and international conferences
- Was responsible for general lab management including ordering reagents, maintaining existing equipment such as cell culture incubators and general lab instruments, setting up new equipment, establishing safety procedures and validating new protocols
Postdoctoral research fellow Sep 2001 - Jun 2010
- Studied the role of cartilage oligomeric matrix protein in cartilage homeostasis and arthritis and the effect of mutations in the dwarfing conditions pseudoachondroplasia and multiple epiphyseal dysplasia
- Contributed ideas and technical expertise for other projects ongoing in the lab
- Wrote manuscripts and grants and served as a reviewer of manuscripts
- Organized and presented work at lab meetings, departmental seminars and also at national and international conferences
Education
University of Manchester 1998 - 2001
Field of study: Biochemistry
Degree: PhD
A study of the molecular pathology of pseudoachondroplasia and multiple epiphyseal dysplasia
University of Manchester 1996 - 1998
Field of study: Biochemistry
Degree: MPhil
Expression of recombinant C-terminal domain of cartilage oligomeric matrix protein
University of Sheffield 1993 - 1996
Field of study: Biochemistry
Degree: BSc Hons
Skills
• Molecular biology/biochemistry:
isolation of genomic DNA, isolation of RNA, cDNA synthesis; primer design, qPCR, RT-PCR, agarose and acrylamide gel electrophoresis of DNA; gel purification and molecular cloning; restriction digestion/mapping; construct design, gene delivery by transformation and transfection; protein expression in bacterial and mammalian cell lines, extraction and purification of tagged and untagged proteins by salt precipitation and various batch/column chromatography (Ion exchange, metal/ligand affinity, HIC, size exclusion); spectrophotometry; Agarose/Acrylamide composite gel analysis of high MW proteins, SDS-PAGE, Silver staining, Zymography, Western blotting (ECL, ECF and LiCor IR); ELISA; immunoprecipitation; immunohistochemistry, confocal microscopy; flow cytometry.
• Cell and tissue culture:
establishment and maintenance of primary cell lines, culture of transformed cell lines and establishment of stably transfected cell lines, adipogenic, chondrogenic and osteogenic differentiation of mesenchymal stem cells
Professional interests
Cartilage biology, Arthritis, Dwarfism, Muscular dystrophy, Bioengineering, Collagen
Fellowships
Characterization of the normal secretory pathway of COMP and the effects of disturbances in the pathological conditions PSACH and MED. 2004 - 2007
Identification of chondrocyte cell surface receptors for cartilage oligomeric matrix protein. 2001 - 2004
Languages
English (Mother tongue), French (Basic)
CV
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