About
Summary
I have Ph.D in Biochemistry and four years of post doctoral experience in biochemistry and structural biology. I have permanent residences (PR) in Canada. I am currently looking for positions within pharmaceutical industries in Canada.
Positions
Postdoc Jan 2012 - Aug 2015
Anatomy and Cell Biology, McGill University
Project summary: Undertook a project to characterize the 3D structure of mammalian coatomer complex I (COPI), which is involved in intra cellular trafficking; using a combination of biochemistry and single particle electron microscopy. The structural data gleaned from this study shed light on its function within the cell.
Duties assumed in this role: Characterized the 3D structure of the protein complex of COPI, using a combination of biochemistry and microscopy.
Established and implemented the protocols; wrote experimental guidelines and laboratory manuals pertinent to the project.
Trained and supervised undergraduate students.
Wrote a grant relevant to the project and secured a fellowship.
Presented talks related to the work at conferences and symposia.
Manuscript, intended for publication in peer reviewed journals in preparation.
Education
Boston University School of Medicine 2004 - 2011
Field of study: Biochemistry
Degree: Ph.D
Expression and biochemical/ biophysical characterization of a soluble construct of the low-density lipooprotein receptor (LDLR) and biophysical characterization of two peptides derived from the cognate ligand to the receptor (Apolipoprotein E).
Skills
Technical Skills:
Expression, purification and biochemical/ biophysical characterization of protein and macromolecular complexes of protein:
PCR, DNA cloning and site-directed mutagenesis, and cell biology techniques – Sf9 cell culture, transfection, and generating competent cells.
Protein expression in bacteria and, Sf-9 (insect) cells.
Protein purification.
Biochemical characterization of protein and protein complexes by Western Blot, SDS-PAGE, Native-PAGE, Blue-Native PAGE, isoelectric focusing, and density gradient ultracentrifugation.
Biophysical characterization by circular dichroism spectroscopy, dynamic light scattering (DLS), small angle x-ray scattering (SAXS) and gradient fixation (GraFix).
Immunological assays: ELISA, ELISPOT and Lymphocyte transformation test.
Structural Analysis of protein/ macromolecular protein complexes by single particle electron microscopy:
Negative stain electron microscopy. Random conical tilt (RCT), and working knowledge of cryo electron microscopy. Knowledge of electron microscopy image processing software like EMAN2, XMIPP, PRIME and RELION. Knowledge of molecular modeling and 3D visualization tools UCSF Chimera, Swiss PDB Viewer, Rasmol, VMD.
Familiar with data analysis packages like Origin and GraphPad.
Known computer languages: C, C++, currently learning Python for EM data analysis, with a strong interest in getting more experience in the computational analysis of EM data.
Transferable skills:
Writing skills: Detailed laboratory manuals, abstracts and posters for conferences, thesis dissertation and manuscript for peer reviewed journal, grant proposal,personal blog and journal, volunteer writer for LeNichoir Wild Bird Conservation Center
Presentation skills: Runs the gamut from informal laboratory meetings to formal talks given at various seminars, symposia and conferences.
Project management: Independent in designing experiments to answer a biological question. Capable of managing finances allotted to the project.
Teaching skills: Teaching assistant for a graduate course in Cell biology, and, trained and mentored undergraduates.
Interpersonal skills: Works well in a team, collaborated with other research groups, multitasking, flexible and adaptable to the challenges of a work day.
Professional interests
R&D within pharmaceutical industries, with an emphasis on protein biochemistry, structural biology and antibody development.
Academic postdocs with emphasis on structural biology of macromolecular protein complexes.
Teaching biochemistry in colleges.
Fellowships
Post Doctoral Trainee Fellowship awarded by CREATE training program in BioNanomachines, by NSERC 2014 - 2015
Awards
Student Travel Bursary Award, Canadian Foundation for the Development of Microscopy 2015
Languages
English
Additional information
Leadership roles:
Vice President Finance for the McGill Anatomy and Cell Biology Graduate Student Society (MAGSS), McGill University 2012-2013.
Member of Graduate advocates for sustainability (GRAS). Involved in raising awareness on sustainability throughout the McGill community, McGill University, 2014.
Volunteer work:
Community service, involving cooking and delivering meals to people with physical and mental challenges.
Outreach service, involving educating underprivileged children.
CV
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Contact details
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