Authors
Creative Biogene
Summary
CRISPR/Cas9 Platform is one of the leading biotechnology companies dedicated to providing customers with CRISPR system solutions to promote scientific research, drug development, disease treatment research, and more. Our scientists have deep pharmacological knowledge and rich experience in experimental operation and data processing.
Introduction
Unlike most dsDNA-targeted CRISPR systems, Cas13 has two advanced eukaryotic and prokaryotic nucleotide-binding (HEPN) domains that target single-stranded RNA (ssRNA). Together they form a ribonuclease active site, enabling it to act as an RNA-guided RRNA endonuclease (as a single-stranded RNA (ssRNA) substrate). In addition, studies show that Cas13 has a unique ribonuclease activity responsible for CRISPR RNA maturation, which is different from its RNA-activated ssRNA degradation activity. The functions of these double ribonucleases differ chemically and mechanically from each other. The two ribonuclease activities of Cas13 enable multiplex processing and direct RNA loading, allowing sensitive cell transcript detection.
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