Authors
Hannah Cole
Summary
DNA microarrays (or DNA chips) have been the most commonly used technique during the last two decades to globally monitor the cellular abundances of transcript species. A DNA microarray is a collection of microscopic DNA spots attached to a solid surface. Each DNA spot contains many thousands of copies of a specific DNA sequence, known as probes.
Introduction
These usually correspond to a short section of a gene generally at the 3′ end. Each microarray contains one or a few probe sets for each interrogated gene. These are used to hybridize a cDNA sample (the target) under high-stringency conditions. Probe–target hybridization is usually detected and quantified by detection of fluorophore-, silver-, or chemiluminescence-labeled targets to determine the relative abundance of transcripts in the target sample. By hybridizing nucleic acid targets including DNA and RNA extracted from a specimen to a DNA microarray, various statuses of DNA and RNA can be analyzed, including determination of nucleic acid sequences of the targets, analysis of gene mutations, measurement of expression levels and copy number of genes, and analysis of methylation status.
Our DNA microarray platform delivers the high quality and consistent arrays, which will give investigators higher confidence in using microarray to study gene expression.
Procedure
cDNA microarrays - uses complementary DNA strands formed by transcription of mRNA
Oligo DNA microarrays - uses chemically synthesized oligo DNA as probes
BAC microarrays - uses template amplified by polymerase chain reaction as the probe
SNP microarrays - used to detect polymorphisms within a population
Anticipated results
Gene expression and discovery
Predicting gene function
Expression profile as a “fingerprint” of cellular or disease phenotype
Detection of mutations and polymorphisms
Linking cell pathways
Drug discovery and drug target validation
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