Authors
Creative Biogene
Summary
Microbial sequencing is performed for a diverse set of applications, such as microbial identification, environmental monitoring, pathogen detection, and routine testing of materials for bacterial contamination. It helps to gain critical genetic insight into bacteria and viruses.
Introduction
• 16S and ITS rRNA Sequencing
16S and Internal Transcribed Spacer (ITS) ribosomal RNA (rRNA) sequencing are common amplicon sequencing methods used to identify and compare bacteria or fungi present within a given sample, and can identify strains that may not be found using traditional methods.
• Shotgun Metagenomics
With the ability to combine many microbial sequencing samples in a single run and obtain high sequence coverage per sample, shotgun metagenomic sequencing can detect very low abundance members of the microbial community that may be missed with other methods.
• Microbial Transcriptome Analysis
Unlike hybridization-based methods such as microarrays, microbial RNA sequencing enables unbiased strand-specific identification of common and novel transcripts.
• Whole-Genome Microbial Sequencing
NGS-based whole-genome sequencing (WGS) allows microbiology researchers to sequence hundreds of organisms with the power of multiplexing. Unlike traditional methods, no labor-intensive cloning steps are needed.
Our sequencing platform serves the genomics research, covering the basic algorithms of bionomics, cloud computing research of biological big data, microbial functional genomics, microbial metagenomics, microbial metatranscriptomics, microbial metaproteomics, biosafety and the environmental uses of microorganisms, and many other frontier research fields.
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