Authors
Creative Bioarray
Summary
In the areas of developmental biology, disease modeling, and cell therapy, research requires experimental approaches that are not only efficient and reproducible but also preserve the genetic integrity of the cells. However, both ordinary cell lines and immortalized cell lines can't meet these special requirements. Stem cells, which have the ability of differentiation into various cell types, provide the possibility of the related research on persistence. Due to some difficulties at the beginning of stem cell research, we design this stem cell culture guide to provide some solutions and resources needed to get your research started.
Introduction
• Stem Cells
The stem cells are a kind of undifferentiated biological cells that have the unique ability to self-renew and differentiate into specialized cell types.
• Classification of Stem Cells
There are two broad types of stem cells: embryonic stem cells and adult stem cells. In adult organisms, stem cells (adult stem cells) normally act as a repair system for the body to replenish adult tissues. Unlike the adult organism, stem cells (embryonic stem cells, ESCs) in a developing embryo could differentiate into all the specialized cells—ectoderm, endoderm, and mesoderm, as well as maintain the normal turnover of regenerative organs, such as blood, skin, or intestinal tissues.
According to different derived from, stem cells can be subdivided into induced pluripotent stem cells(iPSCs), embryonic stem cells (ESCs), mesenchymal stem cells (MSCs), cancer stem cells (CSCs), neural stem cells (NSCs), and hematopoietic stem cells (HSCs), etc.
• Sources of Stem Cells
There are three main sources of human ESC: already existing embryonic stem cell lines; embryos that are left unused after in vitro fertilization procedures (the so-called “spare” embryos); embryos created by means of somatic cell nuclear transfer technique for the purpose of conducting research.
Compared with ESC, adult stem cells can be easier got from a variety of sources including blood, bone marrow, umbilical cord blood, and adipose tissue.
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