Strain Anaerobic Culture

Authors

Jon

Summary

Bacterial strain anaerobic culture is widely used in fields of health, pharmacy, and industry. Many anaerobic strains play a significant role in body growth and diseases. In some experiments, specific bacteria are needed for animal gavage and other treatments. At this time, a large number of specific anaerobic bacteria are in need. Besides, anaerobic bacteria can also be used to develop new energy sources. Various places are promoting the use of anaerobic biological decomposition of organic matter contained in sewage and waste to produce hydrocarbon gas that can be used as fuel. Above all, we can see the importance of the anaerobic culture of strains.

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Introduction

Anaerobic bacteria are a type of bacteria that grow better under anaerobic conditions than in the aerobic environment, but cannot grow on the surface of solid media with air (18% oxygen) and/or 10% carbon dioxide concentration. Such bacteria lack a complete metabolic enzyme system, and their energy metabolism is carried out by anaerobic fermentation.

Anaerobic bacteria are widely distributed in nature, humans, and animals. Non-spore anaerobes mainly exist in human and animal bodies, especially in the intestines, oral cavity, upper respiratory tract, and urinary tract. Together with aerobic bacteria and facultative anaerobes, they form the body's normal flora. Anaerobic bacteria usually have an absolute advantage in normal flora. Under normal circumstances, the protection of the flora is relatively balanced, such as long-term use of broad-spectrum antibiotics, hormones, immunosuppressants, etc. However, the occurrence of flora imbalance or decreased body resistance can lead to endogenous anaerobic bacteria infection. The toxins produced by some anaerobic bacteria are extremely dangerous to advanced organisms including humans (such as tetanus toxin and botulinum toxin).

Strain anaerobic culture refers to the culture of microorganisms in an oxygen-free environment. The techniques include the anaerobic tank method, anaerobic bag method, biological consumption method, pyrogallic acid method, blister medium method, etc.

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