Authors
Microbialtec
Summary
Several methods have been successfully used for the preservation of microorganisms: repeated sub-culturing, preservation on agar beads, oil overlay of slant-grown cultures, use of silica gel and other sterile supports, cryopreservation, and lyophilization. Among these, cryopreservation and lyophilization are highly utilized for culture collections and industry.
Introduction
In addition to the isolation and cultivation of pure strains, adequate preservation is also necessary. A wide variety of techniques are available for bacteria preservation. The primary aim of culture preservation is to maintain the organism alive, uncontaminated, and without variation or mutation, that is, to preserve the culture in a condition that is as close as possible to the original isolate.
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