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Custom Lentivirus Production

Other Created on 29 Mar 2022

Authors

Creative Biogene

Summary

Lentiviral expression vectors are the most effective tools for transducing and stably expressing different effector molecules (cDNA, DNA fragments, siRNA, antisense, ribozymes, etc.) or reporter constructs in virtually any mammalian cell, including nondividing cells and whole model organisms. Like standard plasmid vectors, it is able to introduce lentiviral constructs in plasmid form into the cells with low-to-medium efficiency and get transient expression of effectors by conventional transfection protocols.

Introduction

Nevertheless, production of recombinant lentiviral vectors is hard and challenging, especially when high titer of recombinant lentiviral vectors (>107 transducing unit (TU)/ml) are required. Through the QVirus™ platform at Creative Biogene, we have been generated more lentiviruses than other popular systems. Lentiviruses generated from QVirus™ platform have high safety profile and high efficiency of gene delivery to almost all cell types and whole model organisms.

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