Authors
Rebecca L. Dryer, Lori R. Covey
Summary
The Chromatin Immunoprecipiation (ChIP) provides a powerful technique for identifying the in vivo association of transcription factors with regulatory elements. However, obtaining meaningful information for promoter interactions is extremely challenging when the promoter is a member of a class of highly homologous elements. Use of PCR primers with small numbers of mutations can limit cross-hybridization with non-targeted sequences and distinguish a pattern of binding for factors with the regulatory element of interest. In this report, we demonstrate the selective in vivo association of NF-κB, p300 and CREB with the human Iγ1 promoter located in the intronic region upstream of the Cγ1 exons in the immunoglobulin heavy chain locus. These methods have the ability to extend ChIP analysis to promoters with a high degree of homology.Further details
The protocol was imported from Springer Protocols. To see the entire protocol, click on the source link, or download the pdf here.Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 281
- Comments 0