Authors
Bryan Zimdahl, Jeevisha Bajaj & Tannishtha Reya
Summary
This protocol describes a strategy to image spindle orientation during cell division in hematopoietic stem and progenitor cells in real time. This live imaging method was a modification of a method previously used to visualize the spindle using epithelial cell lines (reference 1). This protocol also describes a strategy to track the orientation of the mitotic spindle coordinately with inheritance of the cell fate determinant Numb in real time.Further details
The protocol was published on Protocol Exchange on 22 April 2014. To see the entire protocol, click on the source link.Advertisement
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