Authors
Midori Ozawa, Yutaka Ozawa, Masashi Iemura, Arihiro Kohara, Kana Yanagihara & Miho K Furue
Summary
In this study, a simple method for the cryopreservation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells is proposed. It is based on the conventional slow-freezing method with 10% DMSO and modified mainly in a thawing protocol without specific equipment or reagents. Recovery rate of the cells cryopreserved by this method was equally high, which is comparable to that of the cells frozen by the vitrification method. In the case of vitrification method, it requires practiced hand because cells can be terribly damaged upon failure of rapid-warming process and strict maintenance of low temperature is required throughout the cryopreservation. On the contrary, our method is available for novices and cryopreserved cells can maintain cell recovery rate for one week after transfer from -150 °C to -80 °C condition. This simple modified method would gain widespread acceptance.Further details
The protocol was published on Protocol Exchange on 27 March 2014. To see the entire protocol, click on the source link.Advertisement
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