Authors
Daisuke Ono, Ken-ichi Honma & Sato Honma
Summary
Photonic bioimaging is a powerful tool for measurement of biological functions in living cells. It enables us to identify when, how, and where a phenomenon of interest takes place such as gene expression and interaction of molecules. To understand the sequential events happening in the brain, it is of special importance to assess more than one parameter simultaneously. In this protocol, we describe detailed methods of simultaneous and long-term measurement of gene expression and neuronal activity from a cultured brain slice. We introduce bioluminescence imaging using CCD camera for measurement of gene expression and multi-electrode array dish for recording of neuronal activity, which enables us to analyze not only functions in a single cell but also cellular communications. The present bimodal recording system reveals spatiotemporal relationship between gene expression and neuronal activity in a single neuron, which promotes us to understand complex phenomena in the brain.Further details
The protocol was published on Protocol Exchange on 13 March 2014. To see the entire protocol, click on the source link.Advertisement
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